HPLC Analysis of Phenolic Antioxidants on Ascentis^® C8

column

Ascentis C8, 15 cm x 4.6 mm I.D., 5 μm particles (581424-U)

mobile phase

[A] 5% acetic acid; [B] (50:50) methanol:acetonitrile

gradient

30 to 100% B in 10 min; held for 5 min

flow rate

2.0 mL/min

column temp.

30 °C

detector

UV, 280 nm

injection

10 μL

sample

as indicated in 50:50 acetonitrile:2-propanol