HPLC Analysis of Poly(ethyleneglycol) polymers on SRT® SEC-150 and SRT® SEC-300
Materials
related product
CONDITIONS
column
SRT-150, 30 cm x 7.8 mm I.D., 5 μm particles, 150 Å (Z777045)
column
SRT-300, 30 cm x 7.8 mm I.D., 5 μm particles, 150 Å (Z777051)
mobile phase
150 mM phosphate Buffer, pH 7.0
flow rate
1.0 mL/min
pressure
720 psi (49.6 bar)
detector
RI(30 C°)
injection
20 μL
sample
1.5 mg/mL in mobile phase PEG 1. MW 636, 6,550, 118,000, 932,000; PEG 2. MW 2010, 42,700, 496,000 ; PEG 3. MW 232, 17,900, 271,000
Description
Analysis Note
Although proteins are the most common applications of Sepax SRT and Zenix gel filtration columns, they can also be used to other water-soluble polymers such as the poly(ethyleneglycol) samples shown in these chromatograms. The conditions are very similar to those used for protein analysis, namely 150mM phosphate buffer at pH 7.0. Judging by the narrow peak widths all PEG polymers shwon here have rather narrow size distributions.
Legal Information
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