HPLC Analysis of Proteins on TSKgel® BioAssist S
Materials
related product
Product No.
Description
Pricing
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell cultureRibonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg proteinTSKgel® BioAssist® S HPLC Column
phase sulfonic acid, L × I.D. 5 cm × 4.6 mm, 7 μm particle size, PEEK hardwareused together
Product No.
Description
Pricing
CONDITIONS
column
TSKgel BioAssist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)
mobile phase
[A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient
0 to 60% in 20 min, 60 to 100% in 0.5 min, held for 5 minutes
flow rate
0.3 mL/min
pressure
400 psi total pressure (28 bar)
column temp.
25 °C
detector
UV, 280 nm
injection
5 μL
sample
ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
Description
Analysis Note
Four commonly investigated proteins were chromatographed on a TSKgel BioAssist S cation exchange column. This column was found to provide better resolution than the Proteomix WCX-NP5, but less resolution between the proteins than the Proteomics SCX-NP5.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation