HPLC Analysis of Purines and Pyrimidines on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 150 x 2.1 mm, 5 μm, 200 Å (1.50454.0001)

mobile phase

[A] acetonitrile; [B] ammonium formate; (80:20, A:B)

flow rate

0.1 mL/min

pressure

(1.4 MPa)

detector

254 nm

injection

2 μL