HPLC Analysis of Pyroglutamic Acid and an Impurity on SeQuant® ZIC-HILIC
CONDITIONS
column
SeQuant ZIC-HILIC, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)
mobile phase
[A] 10mM potassium phosphate pH 3; [B] acetonitrile
gradient
82% B held for 10 min; to 60% B in 10 min; held at 60% B for 5 min
flow rate
1.0 mL/min
pressure
696 psi (48 bar)
detector
210 nm
injection
5 μL
sample
1 mg/mL in mobile phase
Description
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany