HPLC Analysis of Sigma Protein Standard Mix on SRT® SEC-500
CONDITIONS
column
Sepax SRT SEC-500, 30 cm x 4.6 mm I.D., 5 μm particles (Z777055)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate
0.3 mL/min
pressure
580 psi (40 bar)
temp.
25 °C
detector
UV, 215 & 280 nm
injection
2.5 μL
sample
0.5 mL of mobile phase added to one vial of Sigma Protein Standard Mix (p/n 69385); filtered 0.45 μm Millex-HV syringe filter (p/n Z227552)
Description
Analysis Note
Protein standards spanning a wide range of molecular weights can reveal the size range best resolved by a given gel filtration column. The Sepax 500Å SEC column provides good resolution of sample components ranging in molecular weight of ~150,000 to >1000,000.
Legal Information
SRT is a registered trademark of Sepax Technologies