HPLC Analysis of Sigma Protein Standard Mix on Zenix® SEC-150
CONDITIONS
column
Sepax Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 μm particles (Z777016)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate
0.3 mL/min
pressure
1175 psi (81 bar)
column temp.
25 °C
detector
UV, 215 & 280 nm
injection
2.5 μL
sample
0.5 mL of mobile phase added to one vial of Sigma Protein Standard Mix; filtered 0.45 μm Millex-HV syringe filter
Description
Analysis Note
Protein standards spanning a wide range of molecular weights can reveal the size range best resolved by a given gel filtration column. The Sepax 150Å SEC column provides good resolution of sample components ranging in molecular weight of <10,000 to ~150,000.
Legal Information
Zenix is a registered trademark of Sepax Technologies