HPLC Analysis of Simvastatine USP on Chromolith^® Performance Flash 25-4.6mm

sample preparation

1.4mg/ml monobasic potassium phosphate in water

column

Chromolith Performance Flash 25-4.6mm (1.51463.0001)

mobile phase

[A] 50% Acetonitril, 50% phosphoric acid and water 1:999; [B] 100% phosphoric acid and Acetonitril 1:999;

gradient

Initial composition:0-4.5min 100%A, 4.6min to 8 min 95%A to 25%A (8min to 11.5min 25%A, 11.6min to 13min 100%A)

flow rate

3 mL/min

pressure

8337 psi (37-58 bar)

column temp.

ambient

detector

238 nm (cell: 11 μL)

injection

5 μL

sample

Lovastatin (1,5mg/ml), Simvastatin (1,5mg/ml) (Diluent: Acetonitril and Buffer 3:2)