HPLC Analysis of Steroids on Ascentis^® C18 (Mobile phase: 55:45)

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] water; [B] acetonitrile; (55,45:A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL

sample

as indicated in 55:45, water:acetonitrile