HPLC Analysis of Steroids on Ascentis® Express C18
Materials
related product
Product No.
Description
Pricing
Epitestosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Methandienone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Nandrolone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Norethandrolone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18 10cm x 2.1mm, 2.7 μm particles (53823-U)
mobile phase
[A] water [B] acetonitrile (70:30, A:B)
flow rate
0.7 mL/min
pressure
5438 psi (375 bar)
column temp.
40 °C
detector
UV, 254 nm
injection
2 μL
sample
0.125 mg/mL each in 37.5/62.5 water/organic solvent
Description
General description
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany