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HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl

HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl application for HPLC

Materials

related product

Product No.
Description
Pricing

Ascentis® Phenyl HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 353 nm

injection

10 μL

sample

50 μg/mL each in 80:20, water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany