HPLC Analysis of Travoprost on Chromolith^® High Resolution RP-18e

column

Chromolith High Resolution RP-18 endcapped, 100 x 4.6 m (1.52022.0001)

mobile phase

[A] acetonitrile; [B] water + 0,1% TFA v/v; (80:20, A:B)

flow rate

1.0 mL/min

column temp.

ambient

detector

210 nm cell: 11 μL

injection

0.5 μL

sample

Travoprost (Fluprostenol Isopropylacetat) (1mg/mL), Travoprost free acid (Fluprostenol) (1mg/mL)