HPLC Analysis of Urea on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 50 x 4.6 mm, 5 μm, 200 Å (1.50451.0001)

mobile phase

[A] acetonitrile; [B] ammonium acetate 5 mM; (90:10, A:B)

flow rate

0.5 mL/min

detector

204 nm

injection

5 μL