HPLC Analysis of Vitamins, Water Soluble, on Discovery^® C8

column

Discovery C8, 15 cm × 4.6 mm I.D., 5 μm particles (59353-U)

mobile phase

[A] 50 mM potassium phosphate, monobasic, pH 4.5 to methanol: [B] 50 mM potassium phosphate, monobasic, pH 4.(30:70) in 12 min

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL