HPLC Analysis of Xiaoyao Wan on Purospher^® STAR RP-18 endcapped

column

Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)

mobile phase

[A] 0.1% H3PO4 aqueous solution; [B] acetonitrile; (85:15, A:B)

flow rate

1 mL/min

pressure

1537 psi (106 Bar)

column temp.

30 °C

detector

230 nm cell: 1 μL/10mm

injection

10 μL

sample

Dilute ethanol solution (dilute 529 mL ethanol with water to 1000 mL). (60 μg/mL (60 ppm) Paeoniflorin in diluent.)