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LC/MS Analysis of Antifungals on Ascentis® Express Phenyl-Hexyl

LC/MS Analysis of Antifungals on Ascentis® Express Phenyl-Hexyl application for HPLC

Materials

related product

Product No.
Description
Pricing

Ascentis® Express Phenyl-Hexyl (2.7 μm) HPLC Column

L × I.D. 10 cm × 2.1 mm, pk of 1 ea

Fluconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Itraconazole solution

2.0 mg/mL (Methanol with 1% 1M HCl), ampule of 1 mL, certified reference material, Cerilliant®

Posaconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Voriconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ketoconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

used together

Product No.
Description
Pricing

Ammonium acetate

LiChropur, eluent additive for LC-MS

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

gradient

[A] 10 mM ammonium acetate; [B] acetonitrile

gradient

2% B for 1 min; to 100% B in 10 min; held at 100% B for 1 min

flow rate

0.3 mL/min

pressure

2930 psi (202 bar)

column temp.

35 °C

detector

MS, ESI+, XIC

injection

0.5 μL

sample

Antifungals, 1-10 μg/mL in 99:1, water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany