LC-MS of Oligonucleotide Mix 6 on a SeQuant® ZIC®-HILIC 200Å 100 x 2.1 mm (3.5µm)
Materials
related product
CONDITIONS
instrument
Agilent LC-UV-MS 1290 Infinity III coupled to 6545XT QTOF
column
SeQuant® ZIC®-HILIC, 3.5 µm, 200Å 100 x 2.1 mm (1.50441)
mobile phase
[A]: Water; [B]: Acetonitrile [C]: 100 mM Ammonium formate in water pH 4.7 [Example Preparation: to 500 mL of water 1.88 mL of formic acid is added and mixed well and this solution is pH adjusted to 4.7 with ammonium hydroxide solution]
gradient
C always at 20%: 0.00 to 7.00 min 13% A, 67%B; 7.00 to23.00 min to 29% A, 51% B; 23.00 to 23.01 min 43% A, 37% B; 23.01 to 27.00 min 43% A, 37%B; 27.00 to 27.01 min to 13%A, 67% B
flow rate
0.6 mL/min
pressure
240 bar
column temp.
50°C
detector
MSD QTOF; Polarity: negative; Spray voltage: 2000V; Capillary temp: 350 °C; Sheath gas: 12 L/min; Aux. gas: 13 L/min; m/z range: 500-3200 m/z
injection
1.00 µL
sample/matrix
Oligonucleotide HPLC Performance Standard Mix - Oligo-6 (PHR8667) dissolved in 20 µL water under gentle vortexing
Description
General description
The Oligonucleotide HPLC Performance Standard Mix (Oligo-6) is a calibration standard with carefully selected oligonucleotides to test and monitor changes in HPLC and LC/MS Systems. The differences in the selectivity of the oligonucleotides make it useful for most chromatographic modes including Ion Pairing-Reversed Phase, Ion Exchange and HILIC.
The oligonucleotides in the mix are dried and between 12-33 nucleotides making it a suitable tool to facilitate development and quality control of therapeutics like antisense oligonucleotides, small interfering RNAs, and aptamers.
Analysis Note
Analysis of Oligo 6 Mix containing:
Oligo-1: TTT TTT TTT TTT - Mer 12 (3588.40 Da)
Oligo-2: TTT TTT TTT TTT 3′mod {Btn Tg} - Mer 12 (4157.98 Da)
Oligo-3: AGC TGT ACT TTT TTT TTT TTT TTT T - Mer 25 (7580.90 Da)
Oligo-4: AGC TGT ACT TTT TTT TTT TTT TTT TTT TTT TTT - Mer 33 (10014.40 Da)
Oligo-5: TGT GAC CAC GTA GAC TGA CT - Mer 20 (6117.04 Da)
Oligo-6: TCT CTC TCT CTC TCT - Mer 15 (4395.90 Da)
on a SeQuant® ZIC®-HILIC column
Other Notes
App_869
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany