Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins
Materials
related product
Ribonuclease A from bovine pancreas
Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg proteinCONDITIONS
column
TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 20 μm particles (843277)
column
TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 30 μm particles (843177)
column
TOYOPEARL Phenyl-650M, 7.5 cm x 7.5 mm, 65 μm particles (819818)
gradient
for the 10 and 30 μm TSKgel columns: 0 to 100% B in 60 min;
gradient
for the 65 μm TOYOPEARL columns: 0 to 100% B in 240 min
flow rate
for the 10 and 30 μm TSKgel columns: 68 cm/hr;
flow rate
for the 10 and 30 μm TSKgel columns: 68 cm/hr;
for the 65 μm TOYOPEARL columns: 272 cm/hr
detector
UV, 280 nm
injection
100 μL containing 1 mg/mL each protein
Description
Analysis Note
TSKgel and TOYOPEARL particles were packed into 7.5 cm x 7.5 mm columns for this comparison. The part numbers referenced are for the bulk packing. Empty glass columns are sold separately.
Other Notes
Data provided by Tosoh Bioscience LLC.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation