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Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume

Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume application for HPLC

Materials

related product

Product No.
Description
Pricing

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

TSKgel® BioAssist® Desalting HPLC Column

phase polyacrylamide, L × I.D. 15 cm × 10 mm, 13 μm particle size, PEEK

TSKgel® BioAssist® Desalting HPLC Column

phase polyacrylamide, L × I.D. 15 cm × 4.6 mm, 15 μm particle size, PEEK

CONDITIONS

column

TSKgel BioAssist DS, 15 cm x 10 mm I.D., 13 μm particles (821464)

mobile phase

50 mM sodium phosphate buffer, pH 6.5

flow rate

3 mL/min 50 mM sodium phosphate buffer, pH 6.5

column temp.

4 °C

detector

UV, 280 nm and refractive index (RI)

injection

10 μL

sample

10 mg/mL ovalbumin in 0.5 M NaCl and 25 mM sodium phosphate buffer, pH 6.5

Description

Other Notes

Data provided by Tosoh Bioscience LLC.

Legal Information

BioAssist is a registered trademark of Tosoh Corporation
TSKgel is a registered trademark of Tosoh Corporation