HPLC Analysis of Betaine from Chinese Lycium on SeQuant® ZIC-HILIC using ELSD Detection
CONDITIONS
column
SeQuant® ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)
mobile phase
[A] methanol; [B] ammonium acetate; (75:25, A,B)
flow rate
1.0 mL/min
detector
ELSD (neb=40°C, evap=30°C, gas=1.6 SLM)
injection
20 μL raw sample diluted by 50% in water
Description
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany