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LC/MS Analysis of Antifungals on Ascentis® Express Biphenyl

LC/MS Analysis of Antifungals on Ascentis® Express Biphenyl application for LC-MS

Materials

related product

Product No.
Description
Pricing

Fluconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Itraconazole solution

2.0 mg/mL (Methanol with 1% 1M HCl), ampule of 1 mL, certified reference material, Cerilliant®

Posaconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Voriconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ketoconazole solution

2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

used together

Product No.
Description
Pricing

Ammonium acetate

LiChropur, eluent additive for LC-MS

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U)

mobile phase

[A] 10 mM ammonium acetate; [B] acetonitrile

gradient

2% B for 1 min; to 100% B in 10 min; held at 100% B for 1 min

flow rate

0.3 mL/min

pressure

2925 psi (201 bar)

column temp.

35 °C

detector

MS, ESI+, XIC

injection

0.5 μL

sample

Antifungals, 1-10 μg/mL in 99:1, water:methanol

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany