LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5
Materials
related product
Ethyl-β-D-glucuronide
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Ethyl sulfate sodium salt
1.0 mg/mL in methanol (as ethyl sulfate), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase
5 mM ammonium formate in 95:5, acetonitrile:water, adjusted to pH 4.0 with formic acid
flow rate
0.4 mL/min
pressure
1200 psi (83 bar)
column temp.
35 °C
detector
MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z
injection
10 μL
sample
ethyl sulfate (2 μg/mL), ethyl-β-D-glucuronide (20 μg/mL) in 98:2, acetonitrile:methanol
Description
Analysis Note
The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode on C18 columns suffers from poor retention due to the compounds′ polar nature. However, by using HILIC mode on an Ascentis Express OH5 column, both retention and MS-compatibility are improved. Cerilliant CRMs provided reliable quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany