LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express RP-Amide
Materials
related product
Ethyl-β-D-glucuronide
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Ethyl sulfate sodium salt
1.0 mg/mL in methanol (as ethyl sulfate), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase
0.1% (v/v) formic acid in water
flow rate
0.4 mL/min
pressure
3066 psi (211 bar)
column temp.
35 °C
detector
MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z
injection
2 μL
sample
2 μg/mL in 99:1, water:methanol
Description
Analysis Note
The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode using conventional C18 chemistries suffers from poor retention due to the compounds′ polar nature. However, polar compound retention is improved by using the Ascentis Express RP-Amide, a polar-embedded phase, while still keeping the reversed-phase mechanism. Cerilliant CRMs provided reliable quantification.
Legal Information
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