LC/MS Analysis of Fourteen Acyl-L-Carnitines on Ascentis® Express OH5
Materials
related product
CONDITIONS
column
Ascentis Express OH5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53764-U)
mobile phase
[A] 50 mM ammonium formate, pH 3; [B] acetonitrile
gradient
2% A for 0.2 min, to 26% A in 14.8 min; to 50% A in 0.1 min; held at 50% A for 4.9 min; to 2% A in 0.1 min
flow rate
0.4 mL/min
pressure
3640 psi (251 bar)
column temp.
30 °C
detector
MS, ESI(+), 120-500 m/z
injection
0.1 μL
sample
each compound, 0.1 ng on column, except carnitine, 0.2 ng on column
Description
Application
Carnitine plays an important role in fatty acid metabolism. A prerequisite for studies on the wide variety of acyl-L-carnitines and their function is the availability of acyl-L-carnitine standards and a reliable and sensitive analytical methodology. LC/MS using hydrophilic interaction liquid chromatography (HILIC) on an Ascentis Express OH5 column is shown here for the separation of free carnitine and acylcarnitines without derivatization.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany