LC/MS Analysis of Phosphatidylethanol Metabolites on Ascentis® Express RP-Amide
Materials
related product
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.0 μm particles (51569-U)
gradient
5 mM ammonium formate in [A] water; [B] methanol; [C] acetonitrile; (10:45:47, A:B:C)
flow rate
0.5 mL/min
pressure
2745 psi (189 bar)
column temp.
50 °C
detector
MS, ESI-, combined SIR: 699.7, 701.7 m/z
injection
2 μL
sample
500 ng/mL in 50:50, water:methanol
Description
Analysis Note
Phosphatidylethanol and its metabolites in blood are markers of ethanol consumption. Shown here is the rapid resolution of the metabolites on an Ascentis® Express RP-Amide column.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany