LC/MS Analysis of thyroid hormones on Titan PFP
Materials
related product
L-Thyroxine (T4) solution
100 μg/mL in methanol with 0.1N NH3, ampule of 1 mL, certified reference material, Cerilliant®3,3′,5-Triiodo-L-thyronine (T3) solution
100 μg/mL in methanol with 0.1N NH3, ampule of 1 mL, certified reference material, Cerilliant®3,3′,5′-Triiodo-L-thyronine (Reverse T3) solution
100 μg/mL in methanol with 0.1N NH3, ampule of 1 mL, certified reference material, Cerilliant®used together
CONDITIONS
column
Titan PFP, 5 cm X 3.0 mm I.D., 120Å, 1.9 μm particles (569682-U)
mobile phase
[A] 100 mM ammonium acetate, pH 4.0 with acidic acid: water (10:90); [B] 100 mM ammonium acetate, pH with acidic acid: acetonitrile (10:90)
gradient
15 to 55% B in 3 min; to 100% B in 0.1 min; held at 100% B for 1 min; to 15% B in 0.2 min; held at 15% B for 1.7 min
flow rate
0.5 mL/min
pressure
1462 psi (101 bar)
column temp.
45 °C
detector
MS, ESI (+) TOF, SIM, (T3 and rT3 m/z 651.8000; T4 m/z 777.7000
injection
4 μL
sample
100 ng/mL in 99:1 water:acetonitrile
Description
General description
The thyroid hormones, triiodothyronine (T3) and its prohormone, thyroxine (T4), are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Thyroxine, or T4, is the thyroid "storage hormone."Triodothyronine, or T3, is the "energy hormone." For the body to use T4, it must first convert it to the active hormone T3, giving energy to every cell in the body. Another critical thyroid hormone is reverse T3 (rT3). Reverse T3 is the body′s “emergency brake.” rT3 is a metabolite of T4 (thyroxine). Typically, when T4 loses an atom of iodine -- a process known as monodeiodination, or T4 to T3 conversion -- it becomes triiodothyronine (T3), the active thyroid hormone. But in some cases, the body conserves energy by converting the T4 instead into rT3, an inactive form of T3 that is incapable of delivering oxygen and energy to the cells, as T3 does. The Titan PFP shows baseline resolution of these three common thyroid hormones in a 6 minute gradient.
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