LC/MS/MS Analysis of Citrinin on Ascentis® Express Phenyl-Hexyl
CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 2.1 mm I.D., 2.7 μm particles (53334-U)
mobile phase
[A] 5mM ammonium acetate with 0.1% formic acid in water, [B] 5 mM ammonium acetate with 0.1% formic acid in methanol
gradient
50% to 95% B in 2 min, held at 95% B for 1 min, to 50% B in 0.1 min, held at 50% B for 0.9 min
flow rate
0.4 mL/min
pressure
3263 psi (225 bar)
column temp.
40 °C
detector
MS/MS ESI(+), MRM, 251.1 > 233.1 (Citrinin), 264.1 > 246.1 (13C-Citrinin)
injection
2 μL
sample
acetonitirle:water (50:50)
Description
Analysis Note
Using Ascentis Express Phenyl-Hexyl, a fast rugged analytical method was established for citrinin.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany