LC/MS/MS Analysis of Fentanyl and Fentanyl Analogs on Ascentis® Express Biphenyl
Materials
related product
Product No.
Description
Pricing
Alfentanil hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Fentanyl solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Norfentanyl oxalate solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Sufentanil Citrate solution
100 μg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Acetyl fentanyl solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Acetyl norfentanyl oxalate solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Remifentanil hydrochloride solution
100 μg/mL in methanol (as free base), certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Biphenyl, 5 cm x 2.1 mm I.D., 2.7 μm particles (64057-U)
mobile phase
[A] 0.1% formic acid in water ; [B]0.1% formic acid in methanol
gradient
20%B to 40%B in 1 min, then to 50%B in 1 min, then to 80%B in 1 min, then hold 80%B for 1min, then to 20% in 0.1 min, then hold 20% B for 1.4 min
flow rate
0.6 mL/min
pressure
5076 psi (350 bar)
column temp.
50 °C (ambient)
detector
MS/MS, ESI(+), MRM (See Figure for transitions)
injection
1 μL
sample
250 pg/mL in 90:10 methanol:water
Description
Analysis Note
Fast and selective method for fentanyl and fentanyl analogs
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany