LC/MS/MS Analysis of Steroids on Ascentis® Express C18
Materials
related product
Product No.
Description
Pricing
Androstenedione solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®11-Deoxycortisol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®21-Deoxycortisol solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Dehydroepiandrosterone (DHEA) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®5α-Dihydrotestosterone (DHT) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®6β-Hydroxytestosterone solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Hydroxyprogesterone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Methyltestosterone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] 0.1% formic acid in water ; [B] methanol
gradient
5%B to 70%B in 3 minutes, then to 90%B in 0.1 minute, hold 90%B for 0.9 minutes
flow rate
0.5 mL/min
pressure
4351 psi (300 bar)
column temp.
40 °C
detector
MS/MS, ESI(+), MRM (See Figure for transitions)
injection
10 μL
sample
10 ng/mL in methanol
Description
Analysis Note
Fast and selective method for steroids.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany