TLC Analysis of DNP Amino Acids on HPTLC CN F254s (analyte set 3)
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate CN F254s (1.16464.0001)
eluent
[A] acetone; [B] methanol; [C] water; (45:10:45, A:B:C) including 0.1 M lithium chloride
migration distance
8 cm
chamber
normal chamber without chamber saturation
sample
0.4 μL
detector
dipped in dichlorofluorescein (0.2% in ethanol) (in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm)