TLC Analysis of Nucleotides and Trimeric 2′,5′-oligoadenylate on HPTLC NH2 F254s
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate NH2 F254s (1.13192.0001, 1.15647.0001)
eluent
[A] methanol; [B] water; (50:40, A:B) containing 0.2 M lithium chloride
migration distance
5 cm
chamber
normal chamber without chamber saturation
in situ-evaluation with TLC/HPTLC scanner (Camag) UV 254 nm