TLC Analysis of Pentapeptides on HPTLC Silica Gel 60 RP-18 F254s (analyte set 2)

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
TLC pre-coated plate RP-18 F 254s (1.15389.0001, 1.15423.0001)
eluent
[A] acetonitrile; [B] 1N hydrochloric acid; (90:10, A:B) containing 0.1 M tetramethylammonium chloride
migration distance
8 cm
chamber
normal chamber without chamber saturation
sample
0.7 μL
detector
(spray reagent: ninhydrin heated up to 1200 C for 10 min)