TLC Analysis of Progesterones on HPTLC CN F254s

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
TLC pre-coated plate CN F 254s (1.16464.0001)
eluent
A] petroleum benzine (40-60°); [B] acetone; [C] water; (a. straight-phase system: (80:20, A:B) b. reversed-phase system: (60:40, B:C) )
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.2 μL
detector
in situ-evaluation with TLC/HPTLC scanner (Camag) UV 248 nm