TLC Analysis of Sex Hormone Derivatives on HPTLC CN F254s
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate CN F 254s (1.16464.0001)
eluent
[A] petroleum benzene (40-600); [B] ethanol; (80:20, A:B)
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.2 μL
detector
Spray reagent: 5% methanolic perchoric acid heated up to 110 °C for 5 min UV 366 nm