TLC Analysis of Steroids on HPTLC DIOL F254s (analyte set 2)

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate DIOL F 254s (1.12668.0001)
eluent
[A] n-hexane; [B] acetone; [C] acetid acid glacial; (90:10:1, A:B:C) containing 0.1 M lithium cloride
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.2 μL
detector
Spray reagent: MnCl2-sulfuric acid heated up to 110° C for 5 min. (in situ evaluation with TLC/HPTLC scanner (Camag) UV 366 nm)