TLC Analysis of Steroids on HPTLC Silica gel 60 RP-18 F254s

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 F 254s (1.13724.0001)
eluent
[A] acetone; [B] water; (60:40, A:B)
migration distance
5 cm
chamber
normal chamber without chamber saturation
sample
0.2 mL
detector
in-situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm