TLC Analysis of Tripeptides by Ion Pair Chromatography on HPTLC Silica Gel 60 RP-18 F254s

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 F 254s (1.15389.0001, 1.15423.0001)
eluent
[A] acetonitrile; [B] 1 N acetic acid; (98:8, A:B) containing 0.05 M octanesulfonic acid sodium salt
migration distance
12 cm
chamber
normal chamber without chamber saturation
sample
0.7 μL
detector
spray reagent ninhydrin, heated to 120 C for 10 min