TLC Analysis of Tripeptides on HPTLC Silica Gel 60 RP-8 F254s (analyte set 2)
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-8 F 254s (1.15388.0001, 1.15424.0001 )
eluent
[A] acetonitrile; [B] buffer solution pH 3.00; (90:10, A:B) containing 0.1 M lithium chloride
migration distance
12 cm
chamber
normal chamber without chamber saturation
detector
spray reagent: ninhydrin, heated to 110 °C for 15 min