TLC Analysis of Uridine and Phosphate Analogs on HPTLC pre-coated plate PEl-Cellulose F

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
1.05725 HPTLC pre-coated plate PEI-Cellulose F (1.05725.0001, 1.05579.0001)
eluent
Acetic acid 1N/ethanol 80/20 (v/v) with addition of 0.5 mol/l lithium chloride
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.4 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 270 nm