TLC Analysis of Water Soluble Vitamins on HPTLC Silica gel 60 RP-18 w F254s

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 F 254s with concentrating zone (1.15498.0001)
eluent
[A] acetonitrile; [B} dichlormethane; {C] water; (90:10:10, A:B:C) containing 0.1 M lithium chloride
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
1 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 270 nm