TLC Analysis of Weak Polar Steroids on HPTLC DIOL F254s

Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate DIOL F 254s (1.12668.0001)
eluent
([A] di-iso-propy; [B] ether; [C] acetone/acetic acid glacial; (80:20:1, A:B:C))
migration distance
7 cm
chamber
normal chamber without chamber saturation
sample
0.2 μL
detector
in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm