TLC Analysis Of Caffeine in energy drinks on HPTLC Silica gel 60 F254 MS-grade
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
sample preparation
Direct application by spraying 6 mm bands with CAMAG ATS4.
plate
1.00934 HPTLC Silica gel 60 F254 MS-grade 20 cm x10 cm (1.00934.0001)
eluent
[A] 2-Propanol; [B] n-Heptane; [C] water; (7:3:1, A:B)
migration distance
5 cm
chamber
normal chamber without chamber saturation
detector
UV 254 nm; Anisaldehyde-sulphuric-acid-reagent (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression; CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA)