UHPLC Analysis of Aldehyde and Ketone DNPH Derivatives on Ascentis® Express C18
Materials
related product
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)
mobile phase
[A] water; [B] acetonitrile; (40:60, A:B)
flow rate
0.8 mL/min
pressure
9520 psi (656 bar)
column temp.
45 °C
detector
UV, 360 nm
injection
0.1 μL
sample
CARB Method 1004 DNPH Mix 2 (47651-U) diluted 10:1 in 40:60, water:acetonitrile
Description
Analysis Note
Environmental contaminants analyzed by this UHPLC separation are a standard mix of aldehydes and kethones as their DNPH derivatives. Formaldehyde is the target compound for US EPA Compendium Method TO-11A and Method 8315, but at least twelve other carbonyl compounds also can be detected and quantified.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany