UHPLC Analysis of Antifungals on Ascentis® Express C18
Materials
related product
Fluconazole solution
2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Itraconazole solution
2.0 mg/mL (Methanol with 1% 1M HCl), ampule of 1 mL, certified reference material, Cerilliant®Posaconazole solution
2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Voriconazole solution
2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Ketoconazole solution
2.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18 5 cm x 3.0 mm I.D., 2.7 μm particles (53811-U)
mobile phase
[A] 5 mM ammonium acetate pH 6.8 unadjusted in 80:20, water:acetonitrile; [B] 5 mM ammonium acetate pH 6.8 unadjusted in 5:95, water:acetonitrile;
gradient
0% B; to 100% B in 3 min; 100% B held for 0.6 min
flow rate
1.0 mL/min
pressure
2790 psi (192 bar)
column temp.
35 °C
detector
UV, 205 nm
injection
1 μL
sample
6 antifungal 50 μg/mL in 70:30, water:methanol
Description
Analysis Note
The extremely rapid (~3 minutes) resolution of six antifungal compounds on an Ascentis Express C18 column is shown in this application. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany