Titan C18, 5 cm x 3.0 mm I.D., 1.9 μm particles (577126-U)
[A] 5 mM ammonium acetate pH 6.8 unadjusted in 80:20, water:acetonitrile; [B] 5 mM ammonium acetate pH 6.8 unadjusted in 5:95, water:acetonitrile;
0% B; to 100% B in 3 min; 100% B held for 0.6 min
1.0 mL/min
4350 psi (300 bar)
35 °C
UV, 205 nm
1 μL
6 antifungal 50 μg/mL in 70:30, water:methanol
The extremely rapid (~3 minutes) resolution of six antifungal compounds on an Titan C18 column is shown in this application. Titan particles feature totally porous, monodisperse silica. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
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