UHPLC Analysis of Catecholamines on Ascentis® Express F5
Materials
related product
Product No.
Description
Pricing
Catecholamine Mix 1 (Epinephrines) solution
1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Catecholamine Mix 2 (Metanephrines) solution
1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Dopamine hydrochloride solution
1.0 mg/mL in methanol with 5% 1 M HCl (as free base), ampule of 1 mL, certified reference material, Cerilliant®used together
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] water; [B] 100 mM ammonium acetate (pH 4.1 with acetic acid); [C] acetonitrile; (88:2:10, A:B:C)
flow rate
0.3 mL/min
pressure
1880 psi (130 bar)
column temp.
60 °C
detector
UV, 250 nm
injection
Catecholamines 25 μg/mL in 90:10, water:methanol
Description
Analysis Note
The analysis of catecholamines in a clinical setting is often for the diagnosis of pheochromocytoma (a type of adrenal tumor) in symptomatic people. The analysis shown here on Ascentis Express F5 provides rapid resolution of five common catecholamines.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany