BIOshell A400 Protein C4; 10 cm x 2.1 mm I.D., 3.4 μm particles (66825-U)
[A] 70:25:5 water (0.1% TFA): acetonitrile (0.1% TFA): 1-butanol (0.1% TFA); [B] 50:45:5 water (0.1% TFA): acetonitrile (0.1% TFA): 1-butanol (0.1% TFA)
0% B to 100% B in 15 min
0.2 mL/min
680 psi (46 Bar)
75 °C
UV, 215 nm
5 μL
Infliximab, 100 μg/mL, water (0.05% TFA)
Infliximab (brand name Remicade) is an immunosuppressive biologic drug used to treat rheumatoid arthritis, psoriatic arthritis, Crohn′s disease, and many other diseases by blocking TNF alpha which prevents an autoimmune reaction. It has a molecular weight of ~150 kDa. The BIOshell A400 Protein C4 UHPLC column yielded an efficient peak for this protein in less than five minutes.
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