BIOshell IgG 1000 Å C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)
[A] 70:25:5, water (0.1% TFA): acetonitrile (0.1% TFA): 1-butanol (0.1% TFA); [B] 50:45:5, water (0.1% TFA): acetonitrile (0.1% TFA): 1-butanol (0.1% TFA)
0% B to 100% B in 15 min
0.2 mL/min
1800 psi (122 bar)
75 °C
UV, 215 nm
5 μL
Infliximab, 100 μg/mL, water (0.05% TFA)
Infliximab (brand name Remicade) is an immunosuppressive biologic drug used to treat rheumatoid arthritis, psoriatic arthritis, Crohn′s disease, and many other diseases by blocking TNF alpha which prevents an autoimmune reaction. It has a molecular weight of ~150 kDa. The BIOshell IgG 1000 Å C4 UHPLC column yielded an efficient peak for this protein in less than five minutes.
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