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UHPLC Analysis of PP242 (2-(4-Amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol) on Ascentis® Express RP-Amide

UHPLC Analysis of PP242 (2-(4-Amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol) on Ascentis® Express RP-Amide application for UHPLC

Materials

related product

Product No.
Description
Pricing

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

P0037

PP242 hydrate

≥98% (HPLC), powder

CONDITIONS

column

Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] water; [B] acetonitrile; (80:20, A:B)

flow rate

0.4 mL/min

pressure

3050 psi (210 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

1 μL

Description

Analysis Note

PP242 is a potent and selective mTOR inhibitor and is a first selective inhibitor that targets ATP domain of mTOR. From a method development standpoint, PP243 is an interesting compound as it has several hydrogen bond donor groups. For this reason, the Ascentis® Express RPA-Amide phase is a good starting point for the separation.

Other Notes

Stock solution prepared in DMSO, 1 mg/mL

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS