UHPLC Analysis of Synthetic Cannabinoid Metabolites on Ascentis® Express C18
Materials
related product
JWH-018 5-Pentanoic acid metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-018 4-Hydroxypentyl metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-073 4-Butanoic acid metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-073 N-(3-hydroxybutyl) metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-073 4-Hydroxybutyl metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®JWH-018 5-Hydroxypentyl metabolite solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)
mobile phase
[A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile; (55:45, A:B)
flow rate
0.8 mL/min
pressure
10800 psi (744 bar)
column temp.
45 °C
detector
UV, 250 nm
injection
0.5 μL
sample
50 μg/mL in 70:30, water:methanol
Description
Analysis Note
This rapid UHPLC analysis on Ascentis Express 2 μm C18 particles resolves six metabolites of synthetic cannabinoids JWH-018 and JWH-073 in less than two minutes. Synthetic cannabinoids are illicit substances that are smoked as components of herbal incense mixtures. In early 2011, the US Drug Enforcement Agency (DEA) placed several of the most popular synthetic cannabinoids, including JWH-018 and JWH-073, on their Schedule 1 list, making the possession or consumption of these compounds illegal.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany